The purpose of a mordant in the gram stain is


Asked by wiki @ in Biology viewed by 136 People


The purpose of a mordant in the Gram stain is to:

make gram-negative cells visible.
prevent the crystal violet from leaving the cells.
make the flagella visible.
make the bacterial cells larger.
remove the simple stain.

Answered by wiki @



Answer:

The purpose of a mordant in the Gram stain is to:

The answer is "prevent the crystal violet from leaving the cells."

Explanation:

Staining techniques are widely used to visualize those components that are  too difficult to see with the use of a light microscope. Staining involves 3 major steps/processes that include: Counter staining (using Safranin), De-colorization (using ethanol/acetone), and, Staining with crystal violet (a water soluble dye). During the staining procedure, the bacteria are bathed in alcohol, which causes the cell walls to shrink. The primary stain of the Gram's method is crystal violet.

A mordant is classically defined as an ion that binds a chemical dye and keeps it in place and it used for intensifying stains in cell or tissue preparations. Iodine mordant is used in Gram's Staining.

Gram staining is performed when the chemical dye crystal violet is mixed with the mordant iodine. Gram staining differentiates bacteria by the chemical and physical properties of their cell walls and it also distinguishes between Gram-positive and Gram-negative bacteria. Gram staining is based on the ability of bacteria cell wall to retaining the crystal violet dye during solvent treatment.

The purpose of a mordant in the Gram stain is to prevent the crystal violet from leaving the cells, in other words, increase the affinity of the primary stain.


Do you know the better answer?

Similar Questions

The cardiac chamber with the thickest wall is the __________

Asked by wiki @ in Health viewed by 126 persons

The cardiac chamber with the thickest wall is the __________.

The shape of this outward bowed ppc is due to

Asked by wiki @ in Business viewed by 126 persons

A nation's production possibilities curve is bowed out from the origin because: A. resources are not equally efficient in producing every good. B. the originator of the idea drew it …

Use a table of values to graph each quadratic function

Asked by wiki @ in Mathematics viewed by 175 persons

PLEASE HELP ASAP A quadratic function models the graph of a parabola. The quadratic functions, y = x2 and y = x2 + 3, are modeled in the graphs of …

For how many pharaohs was each pyramid constructed

Asked by wiki @ in History viewed by 64 persons

For how many pharaohs was each pyramid constructed?

The land area that supplies water to a river system

Asked by wiki @ in Biology viewed by 177 persons

The land area that supplies water to a river system is called a divide. true or false

What did pinocchio say to his barber math worksheet answers

Asked by wiki @ in Mathematics viewed by 800 persons

What did Pinocchio say to his barber

Can you drive to school with a permit in florida

Asked by wiki @ in Health viewed by 104 persons

In florida if you have a learners permit you may only drive at what time during the first three months

When the federal reserve buys bonds through open market operations

Asked by wiki @ in Law viewed by 212 persons

Through open market operations, the Federal Reserve buys and sells government securities to influence the supply of bank reserves. When the Fed wants to increase excess reserves held by banks, …

How did mom and dad find out about rodricks party

Asked by wiki @ in English viewed by 149 persons

Question 2 of 10 What did Dad do to make sure he wasn't home when Rodrick's band practiced? A He helped coach Greg and Rowley's weekend soccer games. B He …

Why did the struggle for equal rights intensify after ww2

Asked by wiki @ in History viewed by 125 persons

Why did the struggle for equal rights intensify after World War II?

What kind of shape and volume does a solid have

Asked by wiki @ in Chemistry viewed by 156 persons

Match the term with the definition. gas solid plasma Liquid takes the shape and volume of an entire container. charged particles that do not have a definite shape or volume. …

North america is moving away from europe at what rate

Asked by wiki @ in Mathematics viewed by 221 persons

The north american and european continents are moving apart at a rate of about 3 cm/y. at this rate how long will it take them to drift 500 km farther …

Describe the mechanical digestion that takes place in the mouth

Asked by wiki @ in Biology viewed by 274 persons

Classify the following as definitions or examples of chemical digestion or mechanical digestion. Because of the nature of this process, some choices will be used more than once. Other choices …

When performing cpr on an unresponsive choking victim what modification

Asked by wiki @ in Health viewed by 147 persons

When performing cpr on an unresponsive choking victim what modification should you incorporate?

Which of the following statements about pop art is true

Asked by wiki @ in Arts viewed by 180 persons

Which of the following statements about pop art is true

What is the difference between muscular strength and muscular endurance

Asked by wiki @ in Biology viewed by 221 persons

Whats the difference between muscular strength and muscular endurance

According to aristotle what are the six elements of drama

Asked by wiki @ in History viewed by 54 persons

According to Aristotle, what are the six elements of drama?

How to tell if an equation is a direct variation

Asked by wiki @ in Mathematics viewed by 129 persons

For questions 1-3 Determine whether the equation represents a direct variation. If it does, find the constant of variation. 1. 2 y = 5x +1 a. Not a direct variation …

One of the major challenges of supply chain management is

Asked by wiki @ in Business viewed by 192 persons

One of the major challenges of supply chain management is forward buying. maintaining the visibility of inventory at acceptable levels at all times. building safety-stock inventory storage bins. pushing inventory …

As a cell becomes larger its

Asked by wiki @ in Biology viewed by 149 persons

As a cell becomes larger, its volume __________. A. stays the same in relationship to its surface area B. increases much faster than its surface area C. increases much slower …

The monthly bank statement has to be compared with the

Asked by wiki @ in Business viewed by 179 persons

The monthly bank statement has to be compared with the: a. credit card statement b. checkbook c. deposits d. check register user: an alternative to paper bank statements is: a. …

Match each school of psychological thought with its founding contributor

Asked by wiki @ in Health viewed by 393 persons

Match each school of psychological thought with its key belief.

The rising action contributes to this implied resolution by showing

Asked by wiki @ in English viewed by 216 persons

Some sections of "Initiation" imply that Millicent will decline the sorority’s invitation. The rising action contributes to this implied resolution by showing her feeling pride in becoming a candidate for …

The three punctuation marks which can end a sentence are

Asked by wiki @ in English viewed by 223 persons

Which three punctuation marks can be used at the end of a sentence

Will social security be paid if the government shuts down

Asked by wiki @ in Social Studies viewed by 137 persons

Will social security be paid if government shuts down

Why did soviets follow stalin so eagerly despite his atrocities

Asked by wiki @ in History viewed by 205 persons

Why did Soviets follow Stalin so eagerly despite his atrocities? A. Because he was depopulating the USSR so there was more foodfor everyoneB. Because starving peasant farmers were …

What motif is addressed in both forms of poetry

Asked by wiki @ in English viewed by 126 persons

What motif is addressed in both forms of poetry? seasons nature greed love Answer is: B. Nature

Which of the following are legal forms of business organization

Asked by wiki @ in Social Studies viewed by 263 persons

Which of the following legal forms of organization is most expensive to​ organize? A. limited partnership B. partnerships C. sole proprietorships D. corporations

How was the cottage industry affected by the industrial revolution

Asked by wiki @ in History viewed by 208 persons

How was the cottage industry affected by the Industrial Revolution? Fewer goods were made by hand. More people worked at home. Fewer people worked in factories. Fewer goods were made …

Explain how bar graphs can show comparisons between 2 topics

Asked by wiki @ in Physics viewed by 192 persons

Explain how bar graphs can show comparisons between 2 topics.

Most viewed questions in Biology


What do biologists do when they visit france math worksheet

Asked by wiki @ in Biology viewed by 2359 persons


A food worker is putting chemicals into clean spray bottles

Asked by wiki @ in Biology viewed by 2149 persons


When a food handler can effectively remove soil

Asked by wiki @ in Biology viewed by 1766 persons



Which is the correct procedure for delivering prepared hot food

Asked by wiki @ in Biology viewed by 1534 persons


Which food safety practice will help prevent biological hazards quizlet

Asked by wiki @ in Biology viewed by 1517 persons


Why shouldn t you let a doctor worksheet answer key

Asked by wiki @ in Biology viewed by 1470 persons



What must food handlers do before taking out the garbage

Asked by wiki @ in Biology viewed by 1450 persons


Where may food workers eat during breaks at work

Asked by wiki @ in Biology viewed by 1169 persons


When evacuating victims and endangered personnel in a severe emergency

Asked by wiki @ in Biology viewed by 945 persons



What do biologists do when they visit france algebra worksheet

Asked by wiki @ in Biology viewed by 867 persons


Ich e6 describes standards that apply to

Asked by wiki @ in Biology viewed by 858 persons


Amoeba sisters video recap answer key

Asked by wiki @ in Biology viewed by 779 persons



How many nadh and fadh2 are produced in krebs cycle

Asked by wiki @ in Biology viewed by 685 persons


Amoeba sisters video select recap properties of water answer key

Asked by wiki @ in Biology viewed by 677 persons